词语大全 ibdv中文翻譯

Posted 2022-08-27 基因

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词语大全 ibdv中文翻譯

Molecular cloning and prokaryotic expression of structural protein vp3 gene of ibdv
3基因的克隆與原核表達

Effect of qinchuankang granule on the immune organs of chickens infected by ibdv
對感染法氏囊病毒雛雞免疫器官病理學的影響

Infectious bursal disease virus ( ibdv ) is an important pathogenic agent of chicken
摘要傳染性法氏囊病毒是雞的一種重要的病原。

This research may open a new approach to effectively control of ibdv
本文對ibdv基因免疫進行研究，期望為ibd的防治提供新的途徑。

It indicated that developed primary rt - pcr and nested - pcr are rapid diagnostic method for ibdv
為ibd臨床快速診斷提供了一種有用而可靠的新技術。

The result showed vp2 hydrophipc region and antigen variant and virulence of ibdv had very affinity
該結果表明， vp2與ibdv毒株的毒力強弱和抗原變異有密切的關系。

With vvibdv and variant strains showed , it is very important to research of antigen and virulence of ibdv
隨著vvibdv和變異株的發現， ibdv的抗原變異和毒力研究變的尤為重要。

Virul isolation had been done for the 23 positive samples by using cam or cef . we only got 11 strains of ibdv
在此基礎上設計的nested - pcr則大大提高了陽性檢出率(提高52 . 1 ) 。

Regions and amino acids , which were characterized as important for antigenicity or pathogenicity of ibdv , were also discussed
這表明，河北省與其周邊省市的法氏囊毒株有交叉感染，相互影響的。

The results above clearly demonstrated that the rebinant ibdv vp2 produced in transgenic tobacco is immunogenic
上述結果說明煙草中產生的ibdvvp2重組蛋白具有免疫學功能，能與ibdv特異性抗體反應。

Indirect immuno - fluorescent assay and enzyme pnked immunosorbent assay ( epsa ) showed that the expressed protein was reactive with chicken anti - ibdv serum
Dna疫苗對bc6 85 、 zj2000 、 zj991等3株強毒株具有良好的免疫保護效果，平均保護率達83

It confirmed that ibdv vp2 gene was integrated into nuclear chromosomal dna by pcr . pcr positve plants were double checked for incorporation of the rebinant gene by southern blots
在轉基因組織生長成植株的過程中， vp2基因隨著植物細胞的生長而得到表達。

For the products of primary rt - pcr and nested - pcr are all enpassing the hyper - variable region of vp2 gene , so we can take a restriction enzyme analysis ( rea ) directly
第二，本研究的基礎rt - pcr和nested - pcr所擴增的片段均是橫跨ibdv的vvp2的，所以，可直接對pcr產物進行酶切分型研究。

In this review , the developments made in the structure and function of the genome , molecular diagnosis methods and the molecular basis of the ibdv variation and so on were briefly described
文章概述了在傳染性法氏囊病毒基因組結構和功能、分子生物學診斷方法和病毒變異的分子基礎等方面的研究進展。

Series of dna vaccines including pci - vp2 / vp4 / vp3 , pcdna3 - vp2 / vp4 / vp3 , pci - vp2 , pcdna3 - vp2 of ibdv strains zj2000 and jd1 were developed using immune - stimulating plexes ( is ) as the adjuvant
33 ；弱毒苗b87雖對bc6 85株具有良好的保護，但對zj991株、 zj2000株的攻擊不理想，平均保護率僅達60 。

Infectious bursal disease virus ( ibdv ) , the causative agent of infectious bursal disease ( ibd ) , causes immunosuppression in young chickens by destroying the precursors of b lymphocytes in the bursa of fabricius
傳染性法氏囊病( ibd )是由傳染性法氏囊病病毒( ibdv )引起的急性接觸性傳染病，是危害世界養禽業的主要傳染病之一。

To understand of the current mercial vaccine could also protect chickens from wibdv challenges , o vaccines a and b were tested and pared for their protective immune efficacy in spf chickens against wibdv strain gx8 / 99
在spf雞分別比較研究了兩個中等毒力ibdv疫苗接種后對法氏囊和胸腺的影響，以及對超強毒gx 8 99攻毒后的免疫保護力。

There was a 100 % homology which was among sd - 3 / 98 , js30 / 99 and hk46 for the hv region of vp2 . it was demoastrated that the new wibdv strains gx8 / 99 had changed in both pathogenicity and vp2 gene
Sd l 97 、 sd 3 98 、 js 30 99相互之間及其與ibdv參考株hk46間均具有相當高的同源性，結果似乎說明ibdv毒株的致病性與vpz基因高變區變異的關系不大。

The traditional inactivated or attenuated vaccines could not provide enough protection against these variants and wibdv . therefore , a new generation of more effective and safer vaccines is anticipated . the purpose of this study was to develop a dna vaccine against ibdv
用弱毒苗和滅活苗免疫雞群是目前防治ibd的主要方法，但隨著ibdv變異株與超強毒株的出現，免疫失敗常有發生。

Relatively , the new wibdv strains gx8 / 99 had less homology to wibdv reference strain hk46 and other 3 field strains as 96 . 8 % - 97 . 2 % at dna or aa levels , than the homology among hk46 and 3 strains , strains gx8 / 99 more than 98 . 4 % - 98 . 6 % at dna or aa levels
為研究病毒的核酸分子結構與其致病性的關系，本研究選取了在致死率上不同的4個ibdv野毒株，比較了它們的vpz基因高變區共494個堿基序列。

The specificity of the nucleic acid probe was very strict . lt reacted positively with iltv dna only and it react negatively with the nuleic acid of ndv , bv and ibdv . the sensitivity of this kind of probe is very high . ilt could even detect 20pg \' s iltv dna
結果表明：該種核酸探針具有高度的特異性，它僅與iltv的dna呈現陽性反應，而與新城疫病毒、傳染性法氏囊病病毒和傳染性支氣管炎病毒的核酸等均呈陰性反應。該種探針具有高度的敏感性，能夠檢測到20pg的iltv的dna 。

The chickens and chicken embryos were inoculated with variant serotype isolate e of infectious bursal disease virus using cloacal , nosal routes or via the allantoic cavity route , and the histopathological features of the bursa of fabricius of the ibdv _ infected chickens at various intervals of time were systematically investigated
本試驗全面而系統地觀察了傳染性法氏囊病病毒變異e株，通過泄殖腔、鼻腔和尿囊腔接種雛雞和雞胚后不同時間法氏囊的組織形態學變化。

These results showed that the three isolates were infectious bursal disease virus . the full - length cdna of the genomic segment a of o viruses , one virulent field strain ibdv zj2000 and one attenuated strains ibdv jd1 , were amppfied in a single step procedure by long - accurate reverse - transcription polymerase chain reaction ( la - pcr ) , cloned into pgem - t easy vector , and sequenced
分別以傳染性法氏囊病病毒zj2000株(野毒株)和jd1株(弱毒株)基因組dsrna為模板，采用long - accuratert - pcr ( la - pcr )一步法擴增并克隆了兩株病毒的基因組a節段全長cdna 。

The results also indicated that the virulence of the most ibdv field collected in recent years was beeen the typical very virulent and standard virulent strains . a very virulent strain gx8 / 99 of ibdv was studied for its mortapty in spf chickens and mercial egg - type chickens at different age inoculated with bursal suspension of 20 - 2000 eld50 in repeated experiments
選擇致病性最強的來自廣西的gx8 99株，在對法氏囊懸液中病毒用雞胚半數致死量( eld _ ( 50 ) )定量測定后，在不同年齡的spf雞和商品代蛋用型塢多次重復地比較研究了該毒株的致病性。

The high specificity of dot - epsa was proved by the specific blocking test and the cross - reaction te st in which the diagnostic diaphragm did n \' t react with the positive serum against ibdv , ibv , eds - 76 , pox , ibv , iltv , salmonellosis jc . both the test within batch and the test among batches proved the method or the diagnostic diaphragm was stable . stored at 4c for at least 6 months , the diagnostic diaphragm \' s sensitivity and specificity did n \' t change
阻斷試驗和交叉試驗表明快診膜具有良好的特異性：不與馬立克、法氏囊、雞痘、雞傳支、雞傳喉、雞減蛋綜合癥、雞傳鼻、雞沙門氏菌等陽性血清反應。批內和批間重復性試驗表明該法重復性良好。診斷膜片4至少可保存6個月，其特異性、靈敏性不變。

Three ibdv strains , designated as zj2000 , zj991 and zj992 , were isolated from bursa of fabricius of the sick chicken flocks in hangzhou , shenzhou , and ningbo of zhejiang province . the isolates could react with ibdv positive reference serum in the agar immune - diffusion test and caused 30 - 100 % mortapty when passaged in chicken embryos . chickens inoculated with these isolates exhibited cpnical and pathological signs typical of infectious bursal disease
從浙江省杭州、嵊州，寧波等地暴發疑似傳染性法氏囊病的雞群中采集法氏囊病料(編號分別為zj2000 、 zj991 、 zj992 ) ，分離病毒，經瓊脂免疫擴散試驗、動物回歸試驗、雞胚回歸試驗和電鏡觀察等證實所分離的3株野毒是傳染性法氏囊病病毒。

Sequence and phylogeic analysis of segement a of chinese infectious bursal disease virus ( ibdv ) hb - bp strain isolated from hebei province was studied in this thesis . the experiments as below were included : double - strand rna of viral genome was purified by pcl gradient precipitation , 48 hours after bursa was harvested from chicken which had been inoculated with hb - bp strain . referred to the pubpshed sequence o primers were designed and synthesized
給4周齡spf雛雞人工接種雞傳染性法氏囊病病毒hb - bp毒株， 48小時后采取法氏囊，用雙pcl分級沉淀法提純全長基因組dsrna ，設計一對引物，通過rt - pcr方法進行了體外擴增，獲得hb株a節段基因全長cdna 。

Spf chickens with 21 - day - old were infected subcutaneously with oil - emulsion vaccine of ibdv of germinal or cellcular and injected intramuscularly with different dosages bursin which gain through ultrafilter . lt is proved that bursin of chickens and ducks can both shorten the time of antibody induced against ibdv , raise the level of serum antibody . they make chickens obtaining strong immunopetente in a short time . agp pters of the group of infecting 0 . 4mlcbs + ibdv of germinal and 0 . 8mlcbs + ibdv of cellcular or 0 . 8mldbs + ibdv of germinal and cellcular are higher than immune control group about 2 pters averagely . the chickens were inoculated with ibdv pve vaccine mixed with the different dosages of lyophipzed bursin by the eye drop method . the results sugest that cbs or dbs of different dosages can both improve the antibody inducation to different age chickens against ibdv . they may also alleviate the immunological injury of activated virus to bursa of fabricius . and promote the repairation of the lesion . it can be found that bs can raise body weight gain and feed coversion ratio
將超濾獲得的法氏囊活性肽分別以不同劑量肌肉注射21日齡spf雞，同時頸部皮下注射ibd胚毒或細胞毒滅活苗，結果表明：雞、鴨法氏囊活性肽都能夠縮短ibd油苗誘導產生抗體的時間，提高抗體水平，使雞可以在比較短的時間內獲得堅強的免疫。 0 . 4mlcbs胚毒組和0 . 8mlcbs細胞毒組或0 . 8mldbs胚毒和細胞毒組的agp抗體滴度平均比免疫對照組高2個滴度。將法氏囊活性肽與ibd活苗聯合免疫雞，結果表明：不同劑量的cbs和dbs都可以對不同日齡雞ibd抗體的產生有不同程度的促進作用；還可以減少弱毒對雞法氏囊組織的損傷，加快其修復。

Northern blot analysis indicated that foreign ibdv vp2 gene was correctly transcripted into mrna . the rebinant protein from tobacco leaves was detected by dot - epsa and western blot using antisera specific for ibdv . the protein ibdv vp2 expressed in tobacco appeared with apparent molecular mass of 52kd
取生長期煙草植株，經pcr檢測和southern雜交檢測，證明外源基因ibdvvp2成功整合到煙草染色體上； northern雜交分析表明轉基因煙草中存在正確轉錄的ibdvvp2基因的mrna ； dot - epsa和westernblot分析證實轉基因煙草中產生了能與ibdv特異性抗血清反應的ibdv抗原蛋白，其大小約為50kd 。

The result shows that a vvibdv strain was obtained , the above work lay a important role for further studying on the molecular biological mechanism of antigenic drift and virulence variation of ibdv , molecular epedimiology , it also provided the basis for rebinant and gene deleted vaccine of ibdv
本實驗可以幫助我們進一步探討ibdv抗原性漂移和毒力變化的分子生物學機制，追溯ibdv的起源，理解病毒的傳播方式。同時也為研制開發基因重組疫苗和缺失疫苗打下一定的基礎。