词语大全 t-dna中文翻譯

Posted 2022-08-22 抗性

篇首语：锲而舍之，朽木不折；锲而不舍，金石可镂。本文由小常识网(cha138.com)小编为大家整理，主要介绍了词语大全 t-dna中文翻譯相关的知识，希望对你有一定的参考价值。

词语大全 t-dna中文翻譯

A single step transformation system for the generation of a large - scale t - dna insertional mutant population of rice was developed
基于水稻大規模t - dna插入突變體庫的建立，我們發展了一個簡單、快速、高效的農桿菌介導的一步轉化系統。

Insertional mutagenesis using t - dna , transposons and plasmids axe monly methods to creating a mutant pbrary . here we summarize these strategies as well as the progress in the functional genomes research
本文分別介紹三種方法的原理及其在功能基因組學研究中的應用和研究進展。

Pcr analysis of resistant seedpngs with nptii gene primers showed that 6 out of 12 seedpngs detected had the 700bp fragment specific to the plasmid pig121 , indicating that t - dna had been integrated into the genome of sweet cherry
L ，負壓的適宜處理是lmmxio次。 6 、通過p r擴增，初步證明證明外源基回己轉入甜櫻桃。

Totally 99 transgenic rice plants from 125 resistant calp of 191 calp were obtained and pcr assay showed that 80 . 3 % of them were positive . the result of southern blotting analysis for primary plants revealed that each transgenic plant contained a average of 2 . 5 copy of t - dna
對抗性植株進行pcr擴增檢測，結果表明有80 . 3的抗性植株為陽性植株； southern雜交結果進一步證明了質粒的t - dna已經整合到水稻的基因組中，拷貝數為1 4個，平均每棵轉化植株有2 . 5個拷貝。

Mature embryo - derived calp of japonica rice ( oryza sativa l . ) cultivars nipponbare were transformed using agrobacterium tumefaciens strain agl1 carrying a binary vector pcas04 harboring the marker gene , neomycin phosphotransferase gene ( nptii ) , driven by a promoter from the ubiquitin gene in maize , a promoterless p - glucuronidase gene near to the left border of t - dna for trapping gene and a strong promoter , rice actin - gb promoter , near to the right border of t - dna as activation tagging . in this system , co - cultivation was simppfied , special selection stages and pre - regeneration stage were omitted , the whole process was almost under continuous pght at 30 ? except co - cultivation and transgenic plants began to generate only after 7 weeks calp were induced
在一步轉化系統中，光照高溫條件下培養的水稻愈傷組織從誘導開始經過4周時間就可以達到轉化實驗的要求，并且簡化、優化了整個共培養過程，省去了一篩、二篩和預分化步驟，只用7周的時間就可以初步得到再生轉化植株；共191塊愈傷組織得到125塊抗性愈傷組織，轉化頻率達到65 . 4 ，最后共得到99棵獨立來源的再生植株，抗性愈傷組織再生頻率達到79 . 2 。

2 . an anther specific chimaeric male sterile gene expression box with a enhanced promoter ( ta29 ) driving coda gene was constructed and the expression box was inserted into binary vector p3301 that contains a l - phosphinothricin ( ppt ) - resistant selective marker gene and - glucuronidase ( gus ) reporter gene in t - dna region
以增強的ta29啟動子驅動克隆的coda基因，構建成花藥特異性嵌合基因表達盒；將此表達盒插入雙元載體p3301 ，構建成以ppt抗性基因為選擇標記，以gus為報告基因的植物表達載體。