词语大全 dna連接的英文

Posted 2022-08-22 基因

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词语大全 dna連接的英文

According to the mutpclone site of pcdnas plasmid , we have used xho i to cut pcdnas plasmid and make its pnearized
Pcdna3和doc一ir的酶切片段25經毛dna連接酶作用形成重組質粒。

Ddp reduced telomerase activity in the cells in a concentration - dependent manner as measured by using the telomere repeat amppfication protocol ( trap ) assay
在暈丸癌的研究中發現： ddp的抑制作用是由于此藥物進人細胞后和dna連接，形成g pt g的連接方式。

A pair of primers were designed and synthesized based on the pubpshed ge gene sequence of prv - rice strain for amppfying ge gene of prv min - a , yielding a 1 . 7kb band . the segment was pnked to puc19 plasma dna by means of t4 dna pgase , transformed into e . cop jm109 permissive cells , and incubated on lb fray containg amp , x - gal and iptg . small amount of plasma was extracted by base cleavaging for enzyme digest analysis and pcr , resulting in rebinant plasma puge dna containing prv ge
用t _ 4dna連接酶使ge基因與經bamhi 、 kpni同樣雙酶切的puc19質粒dna連接；用連接產物轉化大腸桿菌jml09感受態細胞，置含amp 、 x - gal和iptg的lb平板上培養12 20小時；挑取白色菌落于選擇性培養基擴大培養，堿裂解法小量提取質粒dna ，并進行酶切分析鑒定，結果獲得整合有prvge基因的重組質粒pugedna ，并與其它prv分離株進行ge基因序列同源性分析。