词语大全 collagen type i中文翻譯
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词语大全 collagen type i中文翻譯
Cell adhesion dramatically increased after 6h by fibronectin and after 12h by collagen type i in monolayer culture
1型細胞為多角形或長校形, actin表達較少并相對聚集; 11型細胞細胞圓形,鋪展良好, 。
Cell adhesion to surface of the substrate is essential to development of the anchorage - dependent cells . only after adhering to surface followed by spreading can cells develop and propferate . most synthetic polymers used as orthopaedic matrix substitute present hydrophobicity , which may correlates to the low degree of cell attachment . modification with cell adhesion protein / peptides can be benificial to the cell adhesion on polymers and then affect the cell propferation and differentiation . cell attachment to substrate is primarily mediated by integrins , a widely expressed family of heterodimeric surface receptors . most extrcellular matrix proteins such as fibronectin , osteopontin , collagen type i , bone sialoprotein and vitronectin contain an arg - gly - asp ( rgd ) sequence which is specific to the fixation of cell membrane receptors pke integrin . the main aim of this research is to measure , assess adhesion , propferation of rabbit marrow stromal cells ( mscs ) on the polymers coated by fibronectin , collagen type i or biotie gen , which includes : ( 1 ) biologic characteristics of rabbit mscs were observed by o types of separating method in primary culture . ( 2 ) adhesion , propferation and differentiation of mscs cultured on polymers coated with biotiegen were assessed . ( 3 ) also , adhesion , propferation and differentiation of mscs were assessed on plga film or porous plga substrates coated with fibronectin , or collagen type i respectively . ( 4 ) bone formation was observed on the porous plga substrates coated with collagen type i in vivo . this research aims to give new way to make novel synthetic bone with cell adhesion and high bone induction capabipties
因此將這些蛋白包被、固定到材料表面,觀察骨組織工程種子細胞mscs細胞的粘附、生長特性是本研究的中心環節,并從以下方面進行探討: ( 1 )采用不同原代細胞分離方法,研究其對mscs細胞的生物學特性影響。 ( 2 )檢測基因勝肽膠對mscs細胞粘附、增殖及分化的影響。 ( 3 )分別采用型膠原及纖維粘連蛋白( fibronectin , fn )包被聚乙醇酸-乳酸共聚物( poly ( 1actide - co - glycopde ) , plga )膜及多孔塊型plga材料,觀察細胞在單層或三維培養狀態下,型膠原及fn對mscs細胞粘附、增殖及向成骨細胞分化效應及能力。
There was no difference in other biologic characteristic of mscs beeen the o separation method , such as cell anchorage ratio and clone formation ratio . ( 2 ) plga film presented uniformity frame with no protuberance and fissure under scanning electron microscopy ( sem ) . big aperture with smooth wall and average 400 m i n size running - through each other was observed in porous plga substrate , around the big aperture there were many round micropores about 5 m size . all of the structure were equal and uniform , which satisfied the further research work . ( 3 ) mscs adhesion at earper time was promoted by biotiegenrafter 3h , cell number was ( 1 . 5 0 . 18 ) 105 in the plga film coated with biotiegen group , which was significantly higher than that in plga film group ( p < 0 . 01 ) and higher than that in coverspp group ( p < 0 . 05 ) , which cell number was ( 1 . 04 0 . 21 ) 105 . after 6h and 12h biotiegen could not promote cell adhesion , and cell propferation and alkapne phosphatase ( alp ) activity were not promoted dramatically during 9 days . ( 4 ) cell adhesion was promoted by fibronectin or collagen type i
G ) i型膠原、纖維粘連蛋白促進細胞增殖,細胞接種后3 、 6 、 gd三個檢測時間點,實驗組細胞均明顯高于對照組。與1型膠原相比,纖維粘連蛋白刺激作用更強。 ) i型膠原、纖維粘連蛋白尚能誘導mscs細胞向成骨細胞分化,不僅表達成骨細胞標志物o 、 alp 、 opnmrna ,而且堿性磷酸酶活性明顯增高,堿性磷酸酶及鈣結節7第四軍醫大學博士學位論文一染色均強陽性, i型膠原組mscs細胞堿性磷酸酶活性較fn組更高,有顯著性差異;同時,兔疫組化染色表明,經纖維粘連蛋白作用的mscs1型膠原表達陽性。
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